製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 25093893
ICC/IF Use at an assay dependent concentration. PubMed: 19501574
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/10000.

ターゲット情報

  • 機能
    Likely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis.
  • 関連疾患
    Defects in DLX3 are a cause of trichodentoosseous syndrome (TDO) [MIM:190320]. TDO is an autosomal dominant syndrome characterized by enamel hypoplasia and hypocalcification with associated strikingly curly hair.
    Defects in DLX3 are the cause of amelogenesis imperfecta type 4 (AI4) [MIM:104510]; also known as amelogenesis imperfecta hypomaturation-hypoplastic type with taurodontism. AI4 is an autosomal dominant defect of enamel formation associated with enlarged pulp chambers.
  • 配列類似性
    Belongs to the distal-less homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI4 antibody
    • Distal less homeo box 3 antibody
    • DLX 3 antibody
    • Dlx3 antibody
    • DLX3 distalless homeobox 3 antibody
    • DLX3_HUMAN antibody
    • Homeobox protein DLX 3 antibody
    • Homeobox protein DLX-3 antibody
    • Homeobox protein Dlx3 antibody
    • TDO antibody
    see all

画像

  • Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab64953 at 1/50 dilution. Right hand panel was also treated with immunising peptide.
  • All lanes : Anti-DLX3 antibody (ab64953) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 32 kDa
    Observed band size: 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.

参考文献

This product has been referenced in:
  • Yang G  et al. BMP-2 induced Dspp transcription is mediated by Dlx3/Osx signaling pathway in odontoblasts. Sci Rep 7:10775 (2017). ICC/IF ; Mouse . Read more (PubMed: 28883412) »
  • Wu LA  et al. Establishment of Immortalized BMP2/4 Double Knock-Out Osteoblastic Cells Is Essential for Study of Osteoblast Growth, Differentiation, and Osteogenesis. J Cell Physiol 231:1189-98 (2016). WB ; Mouse . Read more (PubMed: 26595646) »

See all 7 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (cell line)
Permeabilization
Yes - 0.1%NP40
Specification
cell line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Oct 22 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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