製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DLST
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Pig, Zebrafish
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 188-423 of Human DLST

  • ポジティブ・コントロール
    • A431 whole cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
  • パスウェイ
    Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.
  • 配列類似性
    Belongs to the 2-oxoacid dehydrogenase family.
    Contains 1 lipoyl-binding domain.
  • 細胞内局在
    Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2-oxoglutarate dehydrogenase complex component E2 antibody
    • Dihydrolipoamide S succinyltransferase (E2 component of 2 oxo glutarate complex) antibody
    • Dihydrolipoamide S succinyltransferase antibody
    • Dihydrolipoamide succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
    • Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody
    • Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
    • Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex mitochondrial antibody
    • Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody
    • Dlst antibody
    • DLTS antibody
    • E2 antibody
    • E2K antibody
    • mitochondrial antibody
    • ODO2_HUMAN antibody
    • OGDC-E2 antibody
    see all

画像

  • IHC image of DLST staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab95946, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-DLST antibody (ab95946) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 49 kDa
    gel concentration: 10%

参考文献

This product has been referenced in:
  • Lacroix M  et al. E4F1 controls a transcriptional program essential for pyruvate dehydrogenase activity. Proc Natl Acad Sci U S A 113:10998-1003 (2016). WB . Read more (PubMed: 27621446) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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