Anti-DLST 抗体 [9F4BD5] (ab110306)
Key features and details
- Mouse monoclonal [9F4BD5] to DLST
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-DLST antibody [9F4BD5]
DLST 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [9F4BD5] to DLST -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified). This information is considered to be commercially sensitive.
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ポジティブ・コントロール
- Human HDFn and HL-60 cells
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
The purity of ab110306 is near homogeneity as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
ポリ/モノ
モノクローナル -
クローン名
9F4BD5 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110306の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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Flow Cyt | (1) |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). -
パスウェイ
Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6. -
配列類似性
Belongs to the 2-oxoacid dehydrogenase family.
Contains 1 lipoyl-binding domain. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 1743 Human
- Omim: 126063 Human
- SwissProt: P36957 Human
- Unigene: 525459 Human
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別名
- 2-oxoglutarate dehydrogenase complex component E2 antibody
- Dihydrolipoamide S succinyltransferase (E2 component of 2 oxo glutarate complex) antibody
- Dihydrolipoamide S succinyltransferase antibody
see all
画像
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Immunocytochemistry image of ab110306-stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). Cells were then incubated with ab110306 at 1 µg/ml for 2 h at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
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HL-60 cells were stained with 1 µg/mL ab110306 (blue) or an equal amount of an isotype control antibody (red) and analyzed by Flow Cytometry.
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ab110306 staining DLST in Human monocyte-derived macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% Goat serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 1% BSA) for 12 hours at 4°C. A Cy2®-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.
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ab110306 staining DLST in Human monocyte-derived macrophages by Flow Cytometry. Cells were prepared by scraoing in PBS and fixation by paraformaldehyde. The sample was incubated with the primary antibody (1/1000 in PBS + 1% BSA) for 60 minutes at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(1/100) was used as the secondary antibody.
Gating Strategy: Dead cells excluded.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab110306 は 2 報の論文で使用されています。
- Leandro J et al. DHTKD1 and OGDH display substrate overlap in cultured cells and form a hybrid 2-oxo acid dehydrogenase complex in vivo. Hum Mol Genet 29:1168-1179 (2020). PubMed: 32160276
- Malty RH et al. A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling. Cell Syst 5:564-577.e12 (2017). PubMed: 29128334