Anti-DLST 抗体 [9F4BD5] (ab110306)

製品の概要

  • 製品名Anti-DLST antibody [9F4BD5]
    DLST 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [9F4BD5] to DLST
  • アプリケーション適用あり: WB, ICC/IF, Flow Cyt, In-Cell ELISA, IPmore details
  • 種交差性
    交差種: Cow, Human, Pig
  • 免疫原

    Full length native protein (purified) corresponding to Pig DLST. Purified α-ketoglutarate dehydrogenase complex (KGDHC) from Porcine heart.

  • ポジティブ・コントロール
    • Human HDFn and HL-60 cells
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 49 kDa.
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
In-Cell ELISA Use a concentration of 4 µg/ml. (0.4 µg/well)
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
  • パスウェイAmino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.
  • 配列類似性Belongs to the 2-oxoacid dehydrogenase family.
    Contains 1 lipoyl-binding domain.
  • 細胞内局在Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2-oxoglutarate dehydrogenase complex component E2 antibody
    • Dihydrolipoamide S succinyltransferase (E2 component of 2 oxo glutarate complex) antibody
    • Dihydrolipoamide S succinyltransferase antibody
    • Dihydrolipoamide succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
    • Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody
    • Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
    • Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex mitochondrial antibody
    • Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody
    • Dlst antibody
    • DLTS antibody
    • E2 antibody
    • E2K antibody
    • mitochondrial antibody
    • ODO2_HUMAN antibody
    • OGDC-E2 antibody
    see all

Anti-DLST antibody [9F4BD5] 画像

  • Immunocytochemistry image of ab110306-stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). Cells were then incubated with ab110306 at 1 µg/ml for 2 h at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
  • HL-60 cells were stained with 1 µg/mL ab110306 (blue) or an equal amount of an isotype control antibody (red) and analyzed by Flow Cytometry.
  • ab110306 staining DLST in Human monocyte-derived macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% Goat serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 1% BSA) for 12 hours at 4°C. A Cy2®-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.

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  • ab110306 staining DLST in Human monocyte-derived macrophages by Flow Cytometry. Cells were prepared by scraoing in PBS and fixation by paraformaldehyde. The sample was incubated with the primary antibody (1/1000 in PBS + 1% BSA) for 60 minutes at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(1/100) was used as the secondary antibody.
    Gating Strategy: Dead cells excluded.

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  • All lanes : Anti-DLST antibody [9F4BD5] (ab110306) at 1/4000 dilution

    Lane 1 : HeLa Cell lysate
    Lane 2 : Hek293T Cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    DyLight®650-conjugated Goat anti-mouse at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa


    Exposure time : 1 second

    This image is courtesy of an anonymous Abreview

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Anti-DLST antibody [9F4BD5] (ab110306) 使用論文

ab110306 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HELA and HEK-T293)
Loading amount 50 µg
Specification HELA and HEK-T293
Gel Running Conditions Reduced Denaturing (10 %)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Mar 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (human MDM)
Specification human MDM
Preparation Cell harvesting/tissue preparation method: SCRAPING
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy dead cells were excluded
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投稿 Feb 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human MDM)
Specification human MDM
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X
Blocking step GOAT SERUM as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Feb 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"