製品の概要

  • 製品名Anti-Dlp antibody
  • 製品の詳細
    Mouse polyclonal to Dlp
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Drosophila melanogaster
  • 免疫原

    Fusion protein:

    GIDAIEIPQKPSNERVLRYCESPSVGTCCTYNMETRMAMQSRQQLEGHTK DQISRM

    , corresponding to amino acids 91/146 of Fruit fly (Drosophila melanogaster) Dlp

  • 特記事項

    Produced from outbred CD1 mice

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーConstituents: 50% Glycerol
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab22009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 85 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 関連性Dlp (Dally like) protein is required for proper axon guidance and visual-system function. It acts as a gatekeeper, ensuring the sharing of Wingless among cells along the dorsoventral axis. Dlp regulates wg morphogen spatial distribution and signaling. It also has a role in Hedgehog signalling in Drosphila embryonic epidermis.
  • 細胞内局在Cell Membrane
  • 参照データベース
    • 別名
      • D glypican B antibody
      • Dally like antibody
      • Dally like protein antibody
      • dlp antibody

    Anti-Dlp antibody 画像

    • All lanes : Anti-Dlp antibody (ab22009) at 1/1000 dilution

      Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
      Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size : 85 kDa

    Anti-Dlp antibody (ab22009) 使用論文

    ab22009 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample Fruit fly (Drosophila melanogaster) Cell (Drosophila S2 R+ cells)
    Specification Drosophila S2 R+ cells
    Permeabilization No
    Fixative Paraformaldehyde
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    投稿 Oct 25 2013

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample Fruit fly (Drosophila melanogaster) Cell (Drosophila S2 R+ cells)
    Specification Drosophila S2 R+ cells
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Oct 24 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"