製品の概要
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製品名Anti-DLL4 antibody
DLL4 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to DLL4
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由来種Rabbit
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アプリケーション適用あり: IHC-P, WB, ICC/IF, ELISAmore details
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種交差性交差種: Mouse, Human
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免疫原
Synthetic peptide:
AWHAPGDDLRPEAL-C
conjugated to KLH, corresponding to amino acids 121-134 of Human Delta4. -
ポジティブ･コントロール
- Recombinant human DLL4 protein (ab84081)
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab7280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/50. | |
| WB | 1/500 - 1/2500. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). The levels of DLL4 in endogenous samples may be low. Abcam has not been successful in generating western blot data using endogenous samples, but can demonstrate the specificity using recombinant proteins and overexpressing cell lysates. Some customers have been successful using ab7280 for these samples - please see published references below. |
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| ICC/IF | Use at an assay dependent concentration. | |
| ELISA | 1/10000 - 1/50000. |
ターゲット情報
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機能Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4.
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組織特異性Expressed in vascular endothelium.
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配列類似性Contains 1 DSL domain.
Contains 8 EGF-like domains. -
ドメインThe Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.
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翻訳後修飾Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
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細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 54567 Human
- Entrez Gene: 54485 Mouse
- Omim: 605185 Human
- SwissProt: Q9NR61 Human
- SwissProt: Q9JI71 Mouse
- Unigene: 511076 Human
- Unigene: 143719 Mouse
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別名
- AOS6 antibody
- Delta 4 antibody
- delta 4 precursor antibody
see all
画像
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Western blot - Anti-DLL4 antibody (ab7280)All lanes : Anti-DLL4 antibody (ab7280) at 1/1000 dilution
Lane 1 : Recombinant human DLL4 at 0.05 µg
Lane 2 : Whole cell lysate from HEK293 over-expressing human DLL4. at 10 µg
Lane 3 : Whole cell lysate from control HEK293 . at 10 µg
Secondary
All lanes : Peroxidase labelled anti-rabbit antibody at 1/40000 dilution
Performed under reducing conditions.
Predicted band size: 74 kDaThis blot was produced under denaturing, reducing conditions. After transfer, the membrane was blocked for half an hour before being incubated overnight at 4°C with rabbit polyclonal to DLL4 (ab7280; diluted 1:1000). Antibody binding was detected using peroxidase labelled anti-rabbit antibody (diluted 1:40,000) for half an hour at room temperature and visualised using ECL development solution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL4 antibody (ab7280)IHC image of ab7280 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7280, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-DLL4 antibody (ab7280)ICC/IF image of ab7280 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7280, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
参考文献
This product has been referenced in:
- Daley-Brown D et al. Type II Endometrial Cancer Overexpresses NILCO: A Preliminary Evaluation. Dis Markers 2017:8248175 (2017). WB, IHC, Real Time PCR ; Human . Read more (PubMed: 28659656) »
- Oon CE et al. Role of Delta-like 4 in Jagged1-induced tumour angiogenesis and tumour growth. Oncotarget 8:40115-40131 (2017). ICC/IF . Read more (PubMed: 28445154) »
レビューと Q&A
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