製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DLL3
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 526-556 of Human DLL3

  • ポジティブ・コントロール
    • WB: A-375 cell lysate; Human brain and liver lysates. IHC-P: Human Brain tissue. ICC/IF: 293 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab103102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/1000. Predicted molecular weight: 65 kDa.
IHC-P 1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm.
  • 関連疾患
    Spondylocostal dysostosis 1
  • 配列類似性
    Contains 1 DSL domain.
    Contains 6 EGF-like domains.
  • ドメイン
    The DSL domain is required for binding to the Notch receptor.
  • 翻訳後修飾
    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Delta Drosophila like 3 antibody
    • Delta like 3 Drosophila antibody
    • Delta like 3 homolog Drosophila antibody
    • Delta like 3 protein antibody
    • Delta like protein 3 precursor antibody
    • Delta-like protein 3 antibody
    • Delta3 antibody
    • Dll3 antibody
    • DLL3_HUMAN antibody
    • Drosophila Delta homolog 3 antibody
    • SCDO1 antibody
    • SCOD1 antibody
    • Spondylocostal dysostosis autosomal recessive antibody
    see all

画像

  • All lanes : Anti-DLL3 antibody (ab103102) at 1/2000 dilution

    Lane 1 : A-375 (human malignant melanoma cell line) cell lysate
    Lane 2 : Human brain lysate
    Lane 3 : Human liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 65 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • 293 (human epithelial cell line from embryonic kidney) cells stained for DLL3 (green) using ab103102 at 1/10 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L). DAPI was used to stain the cell nuclear (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue sections labeling DLL3 with ab103102 at dilution of 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Anti-DLL3 antibody (ab103102) at 1/1000 dilution + A-375 whole cell lysate at 35 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 65 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

参考文献

This product has been referenced in:
  • Zhang P  et al. Chemotherapy enhances tumor vascularization via Notch signaling-mediated formation of tumor-derived endothelium in breast cancer. Biochem Pharmacol 118:18-30 (2016). WB ; Human . Read more (PubMed: 27520484) »
  • Hu W  et al. Notch3 pathway alterations in ovarian cancer. Cancer Res 74:3282-93 (2014). Read more (PubMed: 24743243) »

See all 2 Publications for this product

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