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CGEEWSQDLHSSGRTDLRYSconjugated to KLH, corresponding to amino acids 155-173 of Mouse Delta-1.
Our Abpromise guarantee covers the use of ab10554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 78 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
Western blot using Delta 1 antibody (ab10554) at 1/500 tested on the following tissue lysates:
Lane 1: Mouse Heart
Lane 2: Mouse Pancreas
Lane 3: Mouse Brain
Lane 4: Rat Heart
Lane 5: Rat Pancreas
Lane 6: Rat Brain
Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 1 minute
Expected molecular weight: 77kDa
Cell lysates at 20
NB: This image shows that is was not possible to detect Delta 1 immunoreactivity by WB in the lysates tested. However little is known about Delta 1 expression and it is likely to be developmentally regulated with low expression/abundance making it difficult to detect in whole tissue homogenates. In favour of Delta 1 antibody (ab10554), the staining on the WB shows very low background and little cross reactivity in whole homogenates.