Anti-DLK-1 抗体 (ab21682)
Key features and details
- Rabbit polyclonal to DLK-1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-DLK-1 antibody
DLK-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DLK-1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Cow, Human -
免疫原
Synthetic peptide corresponding to Human DLK-1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab23442) -
ポジティブ・コントロール
- This antibody gave a positive signal in E18.5 Mouse Muscle Wild Type Tissue Lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21682の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000. Detects a band of approximately 45,48 kDa (predicted molecular weight: 41 kDa).
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product. |
特記事項 |
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WB
1/2000. Detects a band of approximately 45,48 kDa (predicted molecular weight: 41 kDa). Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product. |
ターゲット情報
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機能
May have a role in neuroendocrine differentiation. -
組織特異性
Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. -
配列類似性
Contains 6 EGF-like domains. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 281117 Cow
- Entrez Gene: 8788 Human
- Entrez Gene: 13386 Mouse
- Entrez Gene: 114587 Rat
- Omim: 176290 Human
- SwissProt: P80370 Human
- SwissProt: Q09163 Mouse
- Unigene: 533717 Human
see all -
別名
- Adipocyte differentiation inhibitor protein antibody
- Brevideltin antibody
- Delta like 1 homolog antibody
see all
画像
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All lanes : Anti-DLK-1 antibody (ab21682) at 1/2000 dilution ((5% Milk blocking))
Lane 1 : E18.5 Mouse Muscle Wild Type Tissue Lysate
Lane 2 : E18.5 Mouse Muscle DLK1 Knockout Tissue Lysate
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 45,48 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa (possible cleavage fragment), 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesThis blot was produced using an 8% Tris-glycine gel under the Tris-glycine buffer system. The gel was run at 120V for 1 hour 15 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab21682 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (31)
ab21682 は 31 報の論文で使用されています。
- Flippo C et al. Precocious Puberty in a Boy With Bilateral Leydig Cell Tumors due to a Somatic Gain-of-Function LHCGR Variant. J Endocr Soc 6:bvac127 (2022). PubMed: 36111273
- Watanabe H et al. Soluble factors released by dedifferentiated fat cells reduce the functional activity of iPS cell-derived cardiomyocytes. Cell Biol Int 45:295-304 (2021). PubMed: 33073424
- de Melo CVB et al. Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest. Front Immunol 12:716314 (2021). PubMed: 34804009
- Araujo AN et al. Comprehensive Assessment of Copy Number Alterations Uncovers Recurrent AIFM3 and DLK1 Copy Gain in Medullary Thyroid Carcinoma. Cancers (Basel) 13:N/A (2021). PubMed: 33435319
- Eze UC et al. Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia. Nat Neurosci 24:584-594 (2021). PubMed: 33723434