製品の概要

  • 製品名Anti-DLK antibody [3A10]
    DLK 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3A10] to DLK
  • アプリケーション適用あり: WB, ELISA, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 174-349 of Human DLK

  • ポジティブ・コントロール
    • Recombinant DLK protein; U251 cells; NIH3T3 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 41 kDa.
ELISA 1/10000.
Flow Cyt 1/200 - 1/400. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200 - 1/1000.

ターゲット情報

  • 機能May have a role in neuroendocrine differentiation.
  • 組織特異性Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue.
  • 配列類似性Contains 6 EGF-like domains.
  • 翻訳後修飾N- and O-glycosylated.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adipocyte differentiation inhibitor protein antibody
    • Brevideltin antibody
    • Delta like 1 homolog antibody
    • Delta like homolog antibody
    • Delta like protein antibody
    • Delta1 antibody
    • DLK 1 antibody
    • DLK antibody
    • DLK-1 antibody
    • DLK1 antibody
    • DLK1_HUMAN antibody
    • FA 1 antibody
    • FA1 antibody
    • Fetal antigen 1 antibody
    • pG2 antibody
    • Preadipocyte factor 1 antibody
    • Pref 1 antibody
    • Pref antibody
    • Pref1 antibody
    • Protein delta homolog 1 antibody
    • Secredeltin antibody
    • ZOG antibody
    see all

Anti-DLK antibody [3A10] 画像

  • Anti-DLK antibody [3A10] (ab119930) at 1/500 dilution + Recombinant DLK protein

    Predicted band size : 41 kDa
  • Immunofluorescence analysis of U251 cells using ab119930 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow cytometric analysis of NIH3T3 cells using ab119930 at 1/200 dilution (green) and negative control (red).
  • ELISA using ab119930.

Anti-DLK antibody [3A10] (ab119930) 使用論文

This product has been referenced in:
  • Li L  et al. Targeted disruption of hotair leads to homeotic transformation and gene derepression. Cell Rep 5:3-12 (2013). ICC/IF ; Mouse . Read more (PubMed: 24075995) »
  • Yu L  et al. Pattern recognition receptor-initiated innate antiviral response in mouse adipose cells. Immunol Cell Biol N/A:N/A (2013). Mouse . Read more (PubMed: 24165978) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry.

I am sorry that as this particular antibody has been tested externally in flow cytometry, and we have no information on which secondary as used.

We have many anti-mouse IgG antibodies that would be suit...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (H9 cell line upon differentiation)
Specification H9 cell line upon differentiation
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

投稿 Oct 22 2014

According to the lab, the isotype is IgG1 kappa. We will update our datasheet with this information.

Gracias por tu respuesta.

Ya he realizado el nuevo pedido completamente gratuito del anticuerpo ab119930. Recibiréis una confirmación automática del mismo con más detalles.

Si necesitáis cualquier ...

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Thank you for calling Abcam last night.

I have spoken to my colleagues about your wish to return the 2 vials of ab21682. Since they were purchased more than 2 weeks ago, we cannot accept them as a return, I am sorry about that. I understand t...

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Thank you for your reply.

I've sent a quotation in a separate email, and please let me know if you'd like to update this at any time.

At this time, I've been unable to find an ascites isotype control that we can add to our catalog, ...

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Thank you for contacting us with your questions.

I've answered these questions below, but if you have any further questions please let me know and I'll be happy to help.

1) A 100 uL vial is $337 US dollars, and we charge a flat over...

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Thank you for your reply.

I can confirm we have the following anti mouse IgG secondary antibodies conjugated to PE which are tested and guaranteed in flow cytometry. I can recommend to review the online datasheets for further information. Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"