The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 10 µg/mg of lysate.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 214 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May play a role at the plasma membrane in the maintenance of the structure of epithelial cells and in the transmission of extracellular signals to the membrane and cytoskeleton.
Highly expressed in the placenta and prostate. Expressed at a lower level in the thyroid, spinal cord, trachea, adrenal gland, skeletal muscle, pancreas, heart, brain, liver and kidney. A short splice product shows more limited expression, being absent from at least the brain.
Belongs to the MAGUK family. Contains 1 CARD domain. Contains 1 guanylate kinase-like domain. Contains 4 PDZ (DHR) domains. Contains 1 SH3 domain.
Cell junction. Cell membrane. Localized at sites of cell-cell contact.
All lanes : Anti-DLG5 antibody (ab86783) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 214 kDa
Exposure time: 3 minutes
Immunoprecipitation - DLG5 antibody (ab86783)
Detection of DLG5 by Western Blot of Immunprecipitate.
ab86783, at 1 µg/ml, staining DLG5 in HeLa whole cell lysate immunoprecipitated using ab86783 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.