Anti-DLG5 抗体 (ab86783)
Key features and details
- Rabbit polyclonal to DLG5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DLG5 antibody
DLG5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DLG5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla -
免疫原
Synthetic peptide corresponding to a region between residues 1325 and 1375 of human DLG5 (NP_004738.3).
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ポジティブ・コントロール
- HeLa whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab86783 was affinity purified using an epitope specific to DLG5 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86783の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 214 kDa.
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IP |
Use at 10 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 214 kDa. |
IP
Use at 10 µg/mg of lysate. |
ターゲット情報
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機能
May play a role at the plasma membrane in the maintenance of the structure of epithelial cells and in the transmission of extracellular signals to the membrane and cytoskeleton. -
組織特異性
Highly expressed in the placenta and prostate. Expressed at a lower level in the thyroid, spinal cord, trachea, adrenal gland, skeletal muscle, pancreas, heart, brain, liver and kidney. A short splice product shows more limited expression, being absent from at least the brain. -
配列類似性
Belongs to the MAGUK family.
Contains 1 CARD domain.
Contains 1 guanylate kinase-like domain.
Contains 4 PDZ (DHR) domains.
Contains 1 SH3 domain. -
細胞内局在
Cell junction. Cell membrane. Localized at sites of cell-cell contact. - Information by UniProt
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参照データベース
- Entrez Gene: 9231 Human
- Omim: 604090 Human
- SwissProt: Q8TDM6 Human
- Unigene: 652690 Human
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別名
- Discs large homolog 5 (Drosophila) antibody
- Discs large homolog 5 antibody
- Discs large protein LP DLG antibody
see all
画像
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All lanes : Anti-DLG5 antibody (ab86783) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 214 kDa
Exposure time: 3 minutes -
Detection of DLG5 by Western Blot of Immunprecipitate.
ab86783, at 1 µg/ml, staining DLG5 in HeLa whole cell lysate immunoprecipitated using ab86783 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab86783 は 1 報の論文で使用されています。
- Baskaran Y et al. Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions. Nat Commun 12:5315 (2021). PubMed: 34493720