The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報Western Blot – Approx 30-35kDa bands seen in Jurkat lysate.
Recommended for use at 1-3µg/ml.
Please note that literature indicates DKK4 is detected as three major immunoreactive species of approx. 40 kDa [form (i)], 30-32 kDa [form (ii)] and 15-17 kDa [form (iii)]. We believe this antibody will recognise 30-32kDa species of DKK4.
機能Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
組織特異性Expressed in cerebellum, T-cells, esophagus and lung.
配列類似性Belongs to the dickkopf family.
ドメインThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
翻訳後修飾Appears to be not glycosylated. Can be proteolytically processed by a furin-like protease.