製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab2342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報Western Blot – Approx 30-35kDa bands seen in Jurkat lysate.
    Recommended for use at 1-3µg/ml.

    Please note that literature indicates DKK4 is detected as three major immunoreactive species of approx. 40 kDa [form (i)], 30-32 kDa [form (ii)] and 15-17 kDa [form (iii)]. We believe this antibody will recognise 30-32kDa species of DKK4.
  • ターゲット情報

    • 機能Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
    • 組織特異性Expressed in cerebellum, T-cells, esophagus and lung.
    • 配列類似性Belongs to the dickkopf family.
    • ドメインThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
    • 翻訳後修飾Appears to be not glycosylated.
      Can be proteolytically processed by a furin-like protease.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Dickkopf 4 antibody
      • Dickkopf gene 4 antibody
      • Dickkopf homolog 4 (Xenopus laevis) antibody
      • Dickkopf homolog 4 antibody
      • Dickkopf related protein 4 antibody
      • Dickkopf-4 antibody
      • Dickkopf-related protein 4 short form antibody
      • Dickkopf4 antibody
      • DKK 4 antibody
      • Dkk-4 antibody
      • DKK4 antibody
      • DKK4_HUMAN antibody
      • hDkk 4 antibody
      • hDkk-4 antibody
      • hDkk4 antibody
      • MGC129562 antibody
      • MGC129563 antibody
      see all

    Anti-DKK4 antibody 画像

    • Ab2342 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

    Anti-DKK4 antibody (ab2342) 使用論文

    ab2342 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab2342.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"