製品の概要
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製品名Anti-Dkk3 antibody
Dkk3 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to Dkk3
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由来種Goat
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特異性This antibody is expected to recognise the 350 aa isoform only.
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アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
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種交差性交差種: Human
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免疫原
Synthetic peptide:
AAAALLGGEEI
, corresponding to C terminal amino acids 340-350 of 350 aa isoform DKK3. -
ポジティブ･コントロール
- 293 lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab2459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.03 - 0.1 µg/ml. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 25029272 | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
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組織特異性Highest expression in heart, brain, and spinal cord.
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配列類似性Belongs to the dickkopf family.
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ドメインThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
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翻訳後修飾N- and O-glycosylated.
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細胞内局在Secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 27122 Human
- Omim: 605416 Human
- SwissProt: Q9UBP4 Human
- Unigene: 292156 Human
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別名
- Dickkopf 3 antibody
- Dickkopf 3 homolog (Xenopus laevis) antibody
- Dickkopf 3 homolog antibody
see all
画像
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Western blot - Anti-Dkk3 antibody (ab2459)ab2459 staining (2µ g/ml) of 293 lysate (RIPA buffer, 30µ g total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence Ab2459 staining (2µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dkk3 antibody (ab2459)This image is courtesy of an anonymous Abreviewab2459 staining DKK3 in human MDA-MB 436 cells by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were paraformaldehyde fixed prior to blocking in 10% casein for 30 minutes at 22°C. The primary antibody was diluted 1/75 and incubated with the sample for 30 minutes at 22°C. A biotin conjugated rabbit polyclonal to goat Ig, diluted 1/200, was used as the secondary.
プロトコール
参考文献
This product has been referenced in:
- Marthandan S et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS One 11:e0154531 (2016). WB . Read more (PubMed: 27140416) »
- Marthandan S et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . Read more (PubMed: 26339636) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"