The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.03 - 0.1 µg/ml.
Use at an assay dependent concentration. PubMed: 25029272
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Highest expression in heart, brain, and spinal cord.
Belongs to the dickkopf family.
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
Western blot - 350 aa isoform DKK3 antibody (ab2459)
Ab2459 staining (2µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 350 aa isoform DKK3 antibody (ab2459)This image is courtesy of an anonymous Abreview
ab2459 staining DKK3 in human MDA-MB 436 cells by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were paraformaldehyde fixed prior to blocking in 10% casein for 30 minutes at 22°C. The primary antibody was diluted 1/75 and incubated with the sample for 30 minutes at 22°C. A biotin conjugated rabbit polyclonal to goat Ig, diluted 1/200, was used as the secondary.
Marthandan S et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS One11:e0154531 (2016).
Read more (PubMed: 27140416) »
Marthandan S et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int2015:731938 (2015).
Read more (PubMed: 26339636) »