製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DKK2
  • 特異性
    DKK2 fragments of ~55 kDa and ~28 kDa can also be detected in rat kidney tissue lysate
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, surrounding amino acid 241 of Mouse DKK2

  • ポジティブ・コントロール
    • Lysate from Jurkat cells, 3T3 cells and rat kidney tissue

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 30% Glycerol, 0.5% BSA, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    ab95274 is affinity purified.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 28 kDa.

ターゲット情報

  • 機能
    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • 組織特異性
    Expressed in heart, brain, skeletal muscle and lung.
  • 配列類似性
    Belongs to the dickkopf family.
  • ドメイン
    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • 翻訳後修飾
    May be proteolytically processed by a furin-like protease.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dickkopf 2 antibody
    • Dickkopf gene 2 antibody
    • Dickkopf homolog 2 antibody
    • Dickkopf related protein 2 antibody
    • Dickkopf WNT signaling pathway inhibitor 2 antibody
    • Dickkopf-2 antibody
    • Dickkopf-related protein 2 antibody
    • Dickkopf2 antibody
    • DKK 2 antibody
    • Dkk-2 antibody
    • Dkk2 antibody
    • DKK2_HUMAN antibody
    • hDkk 2 antibody
    • hDkk-2 antibody
    • hDkk2 antibody
    see all

画像

  • Lane 1 : Anti-DKK2 antibody (ab95274) at 4 µg/ml
    Lanes 2 - 4 : Anti-DKK2 antibody (ab95274) at 4 µg/ml (in 1X TBS/T with 5% nonfat milk, overnight at 4C.)

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 3T3 cell lysate
    Lane 4 : Rat kidney tissue lysate

    Secondary
    Anti-rabbit conjugated secondary antibody

    Predicted band size : 28 kDa

プロトコール

参考文献

This product has been referenced in:

See 1 Publication for this product

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