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RabMAb

Anti-DKK1 抗体 [EPR4759] (ab109416)

製品の概要

  • 製品名
    Anti-DKK1 antibody [EPR4759]
    DKK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4759] to DKK1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide within Human DKK1 aa 250-350. The exact sequence is proprietary.

  • ポジティブ・コントロール
    • JAR, HepG2, HeLa, and NCCIT cell lysates; Human placenta tissue; HeLa cells.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa).
IHC-P 1/250 - 1/500. antigen retrieval is recommended.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
    • 組織特異性
      Placenta.
    • 配列類似性
      Belongs to the dickkopf family.
    • ドメイン
      The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Dickkopf 1 antibody
      • Dickkopf 1 homolog antibody
      • Dickkopf 1 like antibody
      • Dickkopf homolog 1 antibody
      • Dickkopf like protein 1 antibody
      • Dickkopf related protein 1 antibody
      • Dickkopf WNT signaling pathway inhibitor 1 antibody
      • Dickkopf-1 antibody
      • Dickkopf-related protein 1 antibody
      • DKK 1 antibody
      • Dkk-1 antibody
      • Dkk1 antibody
      • DKK1_HUMAN antibody
      • hDkk 1 antibody
      • hDkk-1 antibody
      • SK antibody
      see all

    画像

    • All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/1000 dilution

      Lane 1 : JAR cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : NCCIT cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 29 kDa
      Observed band size: 38 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemical analysis of paraffin-embedded Human placenta tissue using 1/100 ab109416
    • Immunofluorescent staining of HeLa cells using 1/100 ab109416

    参考文献

    This product has been referenced in:
    • You S  et al. Tunicamycin inhibits colon carcinoma growth and aggressiveness via modulation of the ERK-JNK-mediated AKT/mTOR signaling pathway. Mol Med Rep 17:4203-4212 (2018). Read more (PubMed: 29344654) »
    • Kulkarni M  et al. Genome-wide analysis suggests a differential microRNA signature associated with normal and diabetic human corneal limbus. Sci Rep 7:3448 (2017). Read more (PubMed: 28615632) »

    See all 10 Publications for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HUVEC)
    Loading amount
    20 µg
    Specification
    HUVEC
    Gel Running Conditions
    Reduced Denaturing (8% gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

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    Verified customer

    投稿 Nov 16 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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