製品の概要

  • 製品名Anti-DKK1 antibody
    DKK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DKK1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human DKK1.

    (Peptide available as ab101729.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Saos 2; HepG2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • 組織特異性Placenta.
  • 配列類似性Belongs to the dickkopf family.
  • ドメインThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dickkopf 1 antibody
    • Dickkopf 1 homolog antibody
    • Dickkopf 1 like antibody
    • Dickkopf homolog 1 antibody
    • Dickkopf like protein 1 antibody
    • Dickkopf related protein 1 antibody
    • Dickkopf WNT signaling pathway inhibitor 1 antibody
    • Dickkopf-1 antibody
    • Dickkopf-related protein 1 antibody
    • DKK 1 antibody
    • Dkk-1 antibody
    • Dkk1 antibody
    • DKK1_HUMAN antibody
    • hDkk 1 antibody
    • hDkk-1 antibody
    • SK antibody
    see all

Anti-DKK1 antibody 画像

  • All lanes : Anti-DKK1 antibody (ab93017) at 1 µg/ml

    Lane 1 : PC3 whole cell lysate (ab3954)
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 29 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-DKK1 antibody (ab93017) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,21 kDa,29 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • IHC image of DKK1 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-DKK1 antibody (ab93017) 使用論文

ab93017 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, Dako, Citrate, Trypsin
Sample Mouse Tissue sections (E10.5, E15.5 embryos)
Specification E10.5, E15.5 embryos
Permeabilization No
Fixative Paraformaldehyde
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投稿 Oct 17 2013

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DISCOUNT CODE: *** Expiration date: February 7th, 2011 I am very pleased to hear you would like to accept our offer and test ab93017 in mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, ple...

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