製品の概要

  • 製品名Anti-DKK1 antibody
    DKK1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to DKK1
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human protein (AAH01539), with a proprietary tag

  • ポジティブ・コントロール
    • WB: HeLa cells lysate. IHC: placenta tissue.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab88334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 29 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • 組織特異性Placenta.
  • 配列類似性Belongs to the dickkopf family.
  • ドメインThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dickkopf 1 antibody
    • Dickkopf 1 homolog antibody
    • Dickkopf 1 like antibody
    • Dickkopf homolog 1 antibody
    • Dickkopf like protein 1 antibody
    • Dickkopf related protein 1 antibody
    • Dickkopf WNT signaling pathway inhibitor 1 antibody
    • Dickkopf-1 antibody
    • Dickkopf-related protein 1 antibody
    • DKK 1 antibody
    • Dkk-1 antibody
    • Dkk1 antibody
    • DKK1_HUMAN antibody
    • hDkk 1 antibody
    • hDkk-1 antibody
    • SK antibody
    see all

Anti-DKK1 antibody 画像

  • Anti-DKK1 antibody (ab88334) at 1 µg/ml + HeLa cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 29 kDa
  • Immunoperoxidase staining of DKK1 using ab88334 at 3 µg/ml on formalin-fixed, paraffin-embedded Human placenta tissue.
  • ICC/IF image of ab88334 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88334, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DKK1 antibody (ab88334) 使用論文

This product has been referenced in:
  • Zhang Z  et al. Differential expression of beta-catenin and dickkopf-1 in the third trimester placentas from normal and preeclamptic pregnancies: a comparative study. Reprod Biol Endocrinol 11:17 (2013). IHC-P ; Human . Read more (PubMed: 23452984) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"