製品の概要

  • 製品名Anti-DKK1 antibody
    DKK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DKK1
  • アプリケーション適用あり: IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) from the internal domain of DKK1 conjugated to KLH

  • ポジティブ・コントロール
    • Human uterus, endometrium.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/200.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C for 20 minutes. Remove from heat and let stand at room temperature in buffer for 20 minutes. Rinse in 1X TBS with Tween (TBST) for 1 minute at RT.

ターゲット情報

  • 機能Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • 組織特異性Placenta.
  • 配列類似性Belongs to the dickkopf family.
  • ドメインThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dickkopf 1 antibody
    • Dickkopf 1 homolog antibody
    • Dickkopf 1 like antibody
    • Dickkopf homolog 1 antibody
    • Dickkopf like protein 1 antibody
    • Dickkopf related protein 1 antibody
    • Dickkopf WNT signaling pathway inhibitor 1 antibody
    • Dickkopf-1 antibody
    • Dickkopf-related protein 1 antibody
    • DKK 1 antibody
    • Dkk-1 antibody
    • Dkk1 antibody
    • DKK1_HUMAN antibody
    • hDkk 1 antibody
    • hDkk-1 antibody
    • SK antibody
    see all

Anti-DKK1 antibody 画像

  • ab61034 at 10µg/ml staining formalin fixed paraffin embedded Uterus (endometrium)
  • ab61034 staining DKK1 in mouse embryonic (E16.5) craniofacial tissue by IHC-Fr (paraformaldehyde-fixed frozen sections).  Paraformaldehyde fixed issue samples were permealized in 0.1% Triton X-100, blocked with 5% serum for 15 minutes at 25 °C. The sample was incubated with primary antibody dilution 1/100 in PBS at 4°C for 24 hours. An Alexa Fluor®  conjugated Goat polyclonal to rabbit IgG (dilution 1/200)  was used as secondary antibody.

    See Abreview

プロトコール

Anti-DKK1 antibody (ab61034) 使用論文

This product has been referenced in:
  • Bleil J  et al. Granulation Tissue Eroding the Subchondral Bone Also Promotes New Bone Formation in Ankylosing Spondylitis. Arthritis Rheumatol 68:2456-65 (2016). Read more (PubMed: 27111225) »
  • Otto B  et al. Molecular changes in pre-metastatic lymph nodes of esophageal cancer patients. PLoS One 9:e102552 (2014). IHC-P ; Human . Read more (PubMed: 25048826) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Skin)
Permeabilization No
Specification Skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Ahmar Aziz

Verified customer

投稿 Jul 08 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH9, 20 min at 100C
Sample Human Tissue sections (colon cancer)
Specification colon cancer
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 30 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, Dako, Citrate, Trypsin
Sample Mouse Tissue sections (E10.5, E15.5 embryos)
Specification E10.5, E15.5 embryos
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Protein-Block Serum Free (DakoCytomation) as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Antigen retrieval step Other
Sample Mouse Tissue sections (mice bone cell)
Specification mice bone cell
Permeabilization No
Fixative 10% neutral-buffered formalin
Username

Abcam user community

Verified customer

投稿 Jun 19 2013

Thank you for your reply. Some other options for positive controls include brain tissue (cerebellum), tonsil, ovary and testis. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results for your customer. The details provided will enable us to investigate this case and will provide us with vital ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Head of Mouse Embryos E16.5)
Specification Head of Mouse Embryos E16.5
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100 (0.1%)
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Gaskon Ibarretxe

Verified customer

投稿 Dec 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"