製品の概要
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製品名Anti-DKFZP434B168 antibody
DKFZP434B168 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to DKFZP434B168
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由来種Rabbit
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アプリケーション適用あり: IHC-P, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human DKFZP434B168 aa 850-900.
Database link: 25896
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab17256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/10000 - 1/25000. Detects a band of approximately 110 kDa. |
ターゲット情報
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機能Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Plays a role in DNA damage response (DDR) signaling during the S phase.
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配列類似性Belongs to the Integrator subunit 7 family.
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細胞内局在Nucleus. Chromosome. Localizes to sites of DNA damage in a H2AX-independent manner.
- Information by UniProt
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参照データベース
- Entrez Gene: 421374 Chicken
- Entrez Gene: 25896 Human
- Omim: 611350 Human
- SwissProt: Q5ZL91 Chicken
- SwissProt: Q1RMS6 Cow
- SwissProt: Q9NVH2 Human
- Unigene: 369285 Human
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別名
- INTS7 antibody
- 5930412E23Rik antibody
- C1orf73 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKFZP434B168 antibody (ab17256)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling DKFZP434B168 with ab17256 at 1/1000 (1µg/ml). Detection: DAB. -
Western blot - Anti-DKFZP434B168 antibody (ab17256)Lane 1 : Anti-DKFZP434B168 antibody (ab17256) at 1/25000 dilution
Lane 2 : Anti-DKFZP434B168 antibody (ab17256) at 1/10000 dilution
All lanes : Nuclear lysates prepared from Hela cells
Observed band size: 110 kDa (why is the actual band size different from the predicted?)Sample: Nuclear extract (NE) from HeLa cells.
Antibody: ab17256 at 0.1 ug/ml. The band corresponding to DKFZP434B168 is arrowed at 110 kD. The identity of the other bands is unknown.
Detection: Chemiluminescence with an exposure time of 15 minutes.
We are unsure about the identity of other bands.
参考文献
ab17256 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"