製品の概要

  • 製品名Anti-DjC8 antibody
    DjC8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DjC8
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region between residue 203 and 253 of Human DjC8 (NP_055095.2).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells and 293T cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab86138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

Anti-DjC8 antibody 画像

  • All lanes : Anti-DjC8 antibody (ab86138) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 30 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa,48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of DjC8 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86138 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.

Anti-DjC8 antibody (ab86138) 使用論文

ab86138 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"