製品の概要

  • 製品名Anti-Dishevelled 2 antibody
    Dishevelled 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Dishevelled 2
  • 特異性ab22616 recognises Dishevelled 2. It does not recognises Dishevelled 1 or Dishevelled 3.
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide:

    (C)GAGRTGRPEERAPES

    conjugated to KLH, corresponding to amino acids 600/614 of Human Dishevelled 2.

  • ポジティブ・コントロール
    • Dishevelled 2 transfected HEK293 cell line.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 10mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)ab22616 is a rabbit antiserum partially purified by caprylic acid and ammonium sulphate precipitation.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab22616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 79 kDa.
Note: dilute ab22616 to working concentration using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used).

ターゲット情報

  • 機能Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
  • 配列類似性Belongs to the DSH family.
    Contains 1 DEP domain.
    Contains 1 DIX domain.
    Contains 1 PDZ (DHR) domain.
  • ドメインThe DIX domain mediates homooligomerization.
  • 細胞内局在Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dishevelled 2 (homologous to Drosophila dsh) antibody
    • Dishevelled dsh homolog 2 antibody
    • dishevelled segment polarity protein 2 antibody
    • Dishevelled-2 antibody
    • Dishevelled2 antibody
    • DSH homolog 2 antibody
    • DVL 2 antibody
    • Dvl2 antibody
    • DVL2_HUMAN antibody
    • Segment polarity protein dishevelled homolog DVL 2 antibody
    • Segment polarity protein dishevelled homolog DVL-2 antibody
    • Segment polarity protein dishevelled homolog DVL2 antibody
    see all

Anti-Dishevelled 2 antibody 画像

  • All lanes : Anti-Dishevelled 2 antibody (ab22616) at 1/1000 dilution

    Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 79 kDa
    Observed band size : 102 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab22616 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22616, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Dishevelled 2 antibody (ab22616) 使用論文

This product has been referenced in:

See 1 Publication for this product

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Vielen Dank für Ihren Anruf.
Mir wurde vom Labor bestätigt, dass die Konzentration dieses Antikörpers leider nicht ermittelbar ist, da er nur teilweise aufgereinigt ist. Eine Proteinmessung würde nicht die Konzentration des Antikörpers messen, son...

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