Anti-DIS3 抗体 (ab68570)
Key features and details
- Mouse polyclonal to DIS3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-DIS3 antibody
DIS3 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to DIS3 -
由来種
Mouse -
特異性
This antibody is reactive against mammalian transfected lysate. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human DIS3. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Constituent: PBS -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68570の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 109 kDa).
|
特記事項 |
---|
WB
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 109 kDa). |
ターゲット情報
-
機能
Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the RNR ribonuclease family.
Contains 1 PINc domain. -
細胞内局在
Cytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Predominantly located in the nucleus. According to PubMed:12429849, found in the nucleolus and according to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3. - Information by UniProt
-
参照データベース
- Entrez Gene: 22894 Human
- Omim: 607533 Human
- SwissProt: Q9Y2L1 Human
- Unigene: 728935 Human
-
別名
- 2810028N01Rik antibody
- bA555G22 antibody
- Dis3 antibody
see all
画像
-
All lanes : Anti-DIS3 antibody (ab68570) at 1/500 dilution
Lane 1 : DIS3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 109 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (3)
ab68570 は 3 報の論文で使用されています。
- Mardakheh FK et al. Proteomics profiling of interactome dynamics by colocalisation analysis (COLA). Mol Biosyst 13:92-105 (2016). PubMed: 27824369
- Pfeiffer JR et al. CARHSP1 is required for effective tumor necrosis factor alpha mRNA stabilization and localizes to processing bodies and exosomes. Mol Cell Biol 31:277-86 (2011). ICC/IF ; Mouse . PubMed: 21078874
- Ayala YM et al. TDP-43 regulates its mRNA levels through a negative feedback loop. EMBO J 30:277-88 (2011). WB ; Human . PubMed: 21131904