製品の概要

  • 製品名
    Anti-Dis3 antibody
  • 製品の詳細
    Rabbit polyclonal to Dis3
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Schizosaccharomyces pombe
  • 免疫原

    Recombinant fragment corresponding to Schizosaccharomyces pombe Dis3.
    Database link: P37202

  • ポジティブ・コントロール
    • S. pombe cell lysates transformed with vector or plasmids carrying truncated genes; S.pombe cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/300. Predicted molecular weight: 110 kDa.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Dis 3 is a catalytic component of the exosome 3'->5' exoribonuclease complex required for the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA and for mRNA decay. Has a 3'-5' exonuclease activity. Implicated in mitotic control. Essential for cell division and spore germination. May be involved in regulating protein dephosphorylation during mitosis.
  • 細胞内局在
    Cytoplasmic and Nuclear
  • 参照データベース
    • 別名
      • 3'-5' exoribonuclease subunit Dis3 antibody
      • Chromosome disjunction protein 3 antibody
      • Exosome complex exonuclease dis3 antibody
      • Mitotic control protein dis3 antibody
      • Ribosomal RNA processing protein 44 antibody
      • rrp44 antibody
      see all

    画像

    • Immunfluorescent analysis of S. pombe cells labeling Dis3 with ab64289 (right panel). DAPI stain for chromosomal DNA (left panel).

    • All lanes : Anti-Dis3 antibody (ab64289) at 1/100 dilution

      Lane 1 : S. pombe cell lysate transformed with the vector
      Lane 2 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (172)
      Lane 3 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (A)
      Lane 4 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (B)
      Lane 5 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (C)
      Lane 6 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (E)


      Predicted band size : 110 kDa

      Polypeptides of expected molecular masses were detected.

    参考文献

    This product has been referenced in:
    • Kinoshita N  et al. The fission yeast dis3+ gene encodes a 110-kDa essential protein implicated in mitotic control. Mol Cell Biol 11:5839-47 (1991). Read more (PubMed: 1944266) »

    See 1 Publication for this product

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