製品の概要
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製品名Anti-DIS antibody
DIS 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to DIS
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由来種Rabbit
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アプリケーション適用あり: WB, IPmore details
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種交差性交差種: Human
交差が予測される動物種: Dog, Chimpanzee, Rhesus monkey, Elephant
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免疫原
Region between residue 1100 and the C terminus (residue 1150) of human DIS
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ポジティブ･コントロール
- Nuclear extract from HeLa cells
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Tris-citrate/phosphate buffer, pH 7 to 8
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Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Antibody was affinity purified using an epitope specific to DIS immobilized on solid support.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab70245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| IP |
追加情報
IP: Use at 1-10µg/mg of lysate.
WB: 1/1000 - 1/10000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/10000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation.
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組織特異性Expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Expression is regulated by growth factors.
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配列類似性Contains 1 SAP domain.
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翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
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細胞内局在Cytoplasm > perinuclear region.
- Information by UniProt
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参照データベース
- Entrez Gene: 450498 Chimpanzee
- Entrez Gene: 479230 Dog
- Entrez Gene: 55749 Human
- Omim: 612569 Human
- SwissProt: Q8IX12 Human
- Unigene: 49853 Human
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別名
- CARP-1 antibody
- Carp1 antibody
- Ccar1 antibody
see all
画像
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Western blot - DIS antibody (ab70245)Lanes 1-2 : Anti-DIS antibody (ab70245) at 1 µg/ml
Lanes 3-4 : Anti-DIS antibody (ab70245) at 0.2 µg/ml
Lanes 1 & 3 : Nuclear extract from HeLa cells at 15 µg
Lanes 2 & 4 : Nuclear extract from HeLa cells at 45 µg
Developed using the ECL technique.
Predicted band size: 132 kDa
Observed band size: 132 kDa
Exposure time: 1 minute -
Immunoprecipitation - DIS antibody (ab70245)Detection of Human CCAR1 by Immunoprecipitation using Nuclear extract (100 µg) from HeLa cells and Affinity purified rabbit anti-CCAR1 ab70245 at 1 µg/100 µg Nuclear extract. Chemiluminescence with exposure times of approximately 3 seconds.
参考文献
ab70245 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"