Anti-DIS 抗体 (ab70243)
Key features and details
- Rabbit polyclonal to DIS
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DIS antibody
DIS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DIS -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan -
免疫原
Synthetic peptide corresponding to a region between N terminal residues 1 and 50 of human DIS. (SwissProt Q8IX12)
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ポジティブ・コントロール
- Nuclear extract from HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Tris-citrate/phosphate buffer, pH 7 to 8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70243の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000 - 1/15000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).
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IP |
Use at 1-10 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/5000 - 1/15000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa). |
IP
Use at 1-10 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation. -
組織特異性
Expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Expression is regulated by growth factors. -
配列類似性
Contains 1 SAP domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm > perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 423692 Chicken
- Entrez Gene: 450498 Chimpanzee
- Entrez Gene: 767587 Cow
- Entrez Gene: 479230 Dog
- Entrez Gene: 55749 Human
- Entrez Gene: 67500 Mouse
- Entrez Gene: 361849 Rat
- Omim: 612569 Human
see all -
別名
- CARP-1 antibody
- Carp1 antibody
- Ccar1 antibody
see all
画像
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All lanes : Anti-DIS antibody (ab70243) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 132 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling DIS with ab70243 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation analysis of whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells (lanes 1 and 2). ab70243 was used for IP at 6 µg/mg lysate. For western blot ab70243 was used at 1 µg/ml. Lane 3 - control IgG.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab70243 は 2 報の論文で使用されています。
- Fei X et al. TRIM22 orchestrates the proliferation of GBMs and the benefits of TMZ by coordinating the modification and degradation of RIG-I. Mol Ther Oncolytics 26:413-428 (2022). PubMed: 36159777
- Li D et al. RP11-284F21.9 promotes lung carcinoma proliferation and invasion via the regulation of miR-627-3p/CCAR1. Oncol Rep 44:1638-1648 (2020). PubMed: 32945522