Anti-Diphtheria Toxin A subunit 抗体 [8A4] (ab8308)

製品の概要

  • 製品名Anti-Diphtheria Toxin A subunit antibody [8A4]
    Diphtheria Toxin A subunit 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [8A4] to Diphtheria Toxin A subunit
  • 特異性This antibody reacts with free A subunits of Diphtheria toxin. It does not react with the whole Diphtheria toxin or free B subunit of Diphtheria toxin
  • アプリケーション適用あり: ELISA, WBmore details
  • 免疫原

    Full length protein.

  • 特記事項

    Detection of Diphtheria toxin A subunit

    For a recombinant antibody to Diphtheria Toxin, please see - Ab209329

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [7F2] to Diphtheria Toxin A subunit (ab8310). Use as capture antibody.
WB Use at an assay dependent concentration.

ターゲット情報

  • 関連性Diphtheria toxin is a 58 kDa protein secreted by lysogenic strains of Corynebacterium diphtheriae. The toxin causes the disease diphtheria in humans by gaining entry into the cell cytoplasm and inhibiting protein synthesis. The mechanism of inhibition involves transfer of the ADP-ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2 inactive. The catalysed reaction is as follows: NAD + + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide The crystal structure of the diphtheria toxin homodimer has been determined to 2.5A resolution. The structure reveals a Y-shaped molecule of 3 domains, a catalytic domain (fragment A), whose fold is of the alpha + beta type; a transmembrane (TM) domain, which consists of 9 alpha-helices, 2 pairs of which may participate in pH-triggered membrane insertion and translocation; and a receptor-binding domain, which forms a flattened beta-barrel with a jelly-roll-like topology. The TM- and receptor binding-domains together constitute fragment B.
  • 別名
    • Diphtheria Toxin antibody

Anti-Diphtheria Toxin A subunit antibody [8A4] (ab8308) 使用論文

ab8308 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 350 µg
Gel Running Conditions Reduced Denaturing
Sample African Green Monkey Cell lysate - whole cell (Kidney Epithelial (vero))
Specification Kidney Epithelial (vero)
Blocking step Licor Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
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Amy

Verified customer

投稿 May 31 2013

The epitopes have not been fully mapped for either of the two antibodies. We do not have a specific ELISA protocol for these and suggest customers use the general protocol on the Abcam homepage.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"