Anti-Diphtheria Toxin A subunit 抗体 [8A4] (ab8308)

製品の概要

  • 製品名
    Anti-Diphtheria Toxin A subunit antibody [8A4]
    Diphtheria Toxin A subunit 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [8A4] to Diphtheria Toxin A subunit
  • 特異性
    This antibody reacts with free A subunits of Diphtheria toxin. It does not react with the whole Diphtheria toxin or free B subunit of Diphtheria toxin
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 免疫原

    Full length protein.

  • 特記事項

    Detection of Diphtheria toxin A subunit

    For a recombinant antibody to Diphtheria Toxin, please see - Ab209329

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [7F2] to Diphtheria Toxin A subunit (ab8310). Use as capture antibody.
WB Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Diphtheria toxin is a 58 kDa protein secreted by lysogenic strains of Corynebacterium diphtheriae. The toxin causes the disease diphtheria in humans by gaining entry into the cell cytoplasm and inhibiting protein synthesis. The mechanism of inhibition involves transfer of the ADP-ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2 inactive. The catalysed reaction is as follows: NAD + + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide The crystal structure of the diphtheria toxin homodimer has been determined to 2.5A resolution. The structure reveals a Y-shaped molecule of 3 domains, a catalytic domain (fragment A), whose fold is of the alpha + beta type; a transmembrane (TM) domain, which consists of 9 alpha-helices, 2 pairs of which may participate in pH-triggered membrane insertion and translocation; and a receptor-binding domain, which forms a flattened beta-barrel with a jelly-roll-like topology. The TM- and receptor binding-domains together constitute fragment B.
  • 別名
    • Diphtheria Toxin antibody

プロトコール

Anti-Diphtheria Toxin A subunit antibody [8A4] (ab8308) 使用論文

ab8308 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
350 µg
Gel Running Conditions
Reduced Denaturing
Sample
African Green Monkey Cell lysate - whole cell (Kidney Epithelial (vero))
Specification
Kidney Epithelial (vero)
Blocking step
Licor Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Username

Amy

Verified customer

投稿 May 31 2013

The epitopes have not been fully mapped for either of the two antibodies. We do not have a specific ELISA protocol for these and suggest customers use the general protocol on the Abcam homepage.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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