製品の概要

  • 製品名Anti-Dio3 antibody
    Dio3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Dio3
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within the C terminal amino acids 249-278 of Human Dio3 (NP_001353).

  • ポジティブ・コントロール
    • HepG2 cell line lysates. Human hepatocarcinoma tissue.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab102926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 34 kDa.Can be blocked with Dio3 peptide (ab131397).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.
  • 組織特異性Expressed in placenta and several fetal tissues.
  • 配列類似性Belongs to the iodothyronine deiodinase family.
  • 細胞内局在Cell membrane. Endosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5DIII antibody
    • D3 antibody
    • Deiodinase iodothyronine type 3 antibody
    • Deiodinase iodothyronine type III antibody
    • Dio 3 antibody
    • Dio3 antibody
    • DIOIII antibody
    • IOD3_HUMAN antibody
    • Iodothyronine deiodinase placental type antibody
    • MGC124117 antibody
    • MGC124118 antibody
    • Thyroxine deiodinase type III antibody
    • Thyroxine deiodinase type III (selenoprotein) antibody
    • TXDI3 antibody
    • Type 3 DI antibody
    • Type 3 iodothyronine selenodeiodinase antibody
    • Type III 5' deiodinase antibody
    • Type III iodothyronine deiodinase antibody
    • Type-III 5''-deiodinase antibody
    see all

Anti-Dio3 antibody 画像

  • Anti-Dio3 antibody (ab102926) at 1/100 dilution + HepG2 cell line lysate at 35 µg

    Predicted band size : 34 kDa
  • ab102926 at 1/50 staining Dio3 in Formalin-fixed and paraffin-embedded Human hepatocarcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection utilised DAB.

Anti-Dio3 antibody (ab102926) 使用論文

ab102926 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Cow Cell (aortic endothelial cells)
Permeabilization Yes - Saponin 0,1%
Specification aortic endothelial cells
Blocking step PBS/Saponin 0,1%/BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative PFA 4%
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投稿 Jul 08 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - other (Heart)
Specification Heart
Blocking step Super Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
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投稿 Jul 30 2014

Thank you for your patience. My colleague has been able to add the following peptides to our catalog:

Ab77481 corresponding peptide Ab131396

Ab102926 corresponding peptide Ab131397

Ab22835 corresponding peptide AB131398 ...

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Thank you for your reply.

I will be working on adding these peptides to our catalog, and it may take a couple of days.

Regardingthe TSH beta antibody ab80784, the immunogen was purified human TSH beta. We don't have the beta subuni...

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Thank you for your enquiry.

I have checked our catalog and unfortunately we do not have a blocking peptide for use withab102926.

I'm sorry I could not be more helpful at this time. Please contact me again if you have any further que...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"