製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to DIO2
  • アプリケーション
    適用あり: WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Rabbit, Goat, Chicken, Cow, Human, Pig
  • 免疫原

    Synthetic peptide:

    EVKKHQNQEDRC

    (Human) from the internal region of the protein sequence according to NP_000784.2; NP_054644.1; NP_001007024.1.

  • ポジティブ・コントロール
    • Human heart and thyroid lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.05 µg/ml. Predicted molecular weight: 31 kDa.Can be blocked with DIO2 peptide (ab131396). Preliminary experiments gave an approx 65-70 kDa band in human heart and thyroid lysates after 0.05 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.5 kDa according to NP_000784.2 and of 34.6 kDa according to NP_001007024.1. The 65-70 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC-Fr Use a concentration of 2 - 3 µg/ml. Tissue sections were PFA fixed.

ターゲット情報

画像

  • ab77481 staining DIO2 in mouse Brain (Hippocampus) tissue section by Immunohistochemistry (Frozen sections). Tissue underwent antigen retrieval in microwave with citrate buffer (pH 4.5). The HRP-staining procedure was used for detection.

  • ab77481 staining DIO2 in DIO2 Brain (Hypothalamus) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/1000) for 48 hours at 4°C. A Biotinylated Horse Anti-Goat IgG polyclonal antibody (1/400) was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Shu L  et al. A-FABP mediates adaptive thermogenesis by promoting intracellular activation of thyroid hormones in brown adipocytes. Nat Commun 8:14147 (2017). WB . Read more (PubMed: 28128199) »
  • Wang YY  et al. Up-regulation of type 2 iodothyronine deiodinase in dilated cardiomyopathy. Cardiovasc Res : (2010). WB ; Mouse . Read more (PubMed: 20453157) »

See all 2 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Red sided garter snake Tissue sections (Brain (Hypothalamus))
Permeabilization
No
Specification
Brain (Hypothalamus)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 06 2017

Thank you for your patience. My colleague has been able to add the following peptides to our catalog:

Ab77481 corresponding peptide Ab131396

Ab102926 corresponding peptide Ab131397

Ab22835 corresponding peptide AB131398 ...

Read More

Thank you for your reply.

I will be working on adding these peptides to our catalog, and it may take a couple of days.

Regardingthe TSH beta antibody ab80784, the immunogen was purified human TSH beta. We don't have the beta subuni...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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