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RabMAb

Anti-Dihydrofolate reductase (DHFR) 抗体 [EPR5285] (ab124814)

製品の概要

  • 製品名
    Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]
    Dihydrofolate reductase (DHFR) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5285] to Dihydrofolate reductase (DHFR)
  • アプリケーション
    適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human Dihydrofolate reductase (DHFR).

  • ポジティブ・コントロール
    • HeLa, Jurkat, Y-79, 293T, Mouse spleen and Rat kidney lysates; Human cervical carcinoma tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC 1/25 - 1/50.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    画像

    • All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814) at 1/10000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : Jurkat cell lysates
      Lane 3 : Y-79 cell lysates
      Lane 4 : 293T cell lysates
      Lane 5 : Mouse spleen lysates
      Lane 6 : Rat kidney lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 21 kDa
    • ab124814 at 1/100 dilution, staining Dihydrofolate reductase (DHFR) in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.

    参考文献

    This product has been referenced in:
    • Allen MD  et al. Prognostic and therapeutic impact of argininosuccinate synthetase 1 control in bladder cancer as monitored longitudinally by PET imaging. Cancer Res 74:896-907 (2014). Read more (PubMed: 24285724) »
    • Garon EB  et al. The HSP90 inhibitor NVP-AUY922 potently inhibits non-small cell lung cancer growth. Mol Cancer Ther 12:890-900 (2013). Read more (PubMed: 23493311) »

    See all 2 Publications for this product

    レビューと Q&A



    This antibody was raised using a synthetic peptide corresponding to the C terminal end of the protein DHFR.

    Thank you for contacting Abcam regarding ab124814.


    For this product we do not have any information regarding storage at 4C. In general we would expect decreased activity over time when stored at this temperature. Furthermore, we do not ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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