Rabbit polyclonal to Dihydrofolate reductase (DHFR)
Mouse, Rabbit, Cow, Pig
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Dihydrofolate reductase (DHFR).
(Peptide available as
This antibody gave a positive signal in Human liver tissue lysate, and in the following whole cell lysates: HepG2; HeLa; Jurkat; MCF7.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Belongs to the dihydrofolate reductase family.
Contains 1 DHFR (dihydrofolate reductase) domain.
Information by UniProt
Dihydrofolate reductase antibody
Western blot - Anti-Dihydrofolate reductase (DHFR) antibody (ab85056)
All lanes :
Anti-Dihydrofolate reductase (DHFR) antibody (ab85056) at 1 µg/ml
Lane 1 :
Human liver tissue lysate - total protein (
Lane 2 :
HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 :
Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 :
MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
Additional bands at:
36 kDa, 42 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-Dihydrofolate reductase (DHFR) antibody (ab85056)
ICC/IF image of ab85056 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85056, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml, and in 100% Methanol fixed (5 min) HepG2, and HeLa cells at 5µg/ml.
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