Western blot - Dihydrofolate reductase (DHFR) antibody (ab49881)
All lanes : Anti-Dihydrofolate reductase (DHFR) antibody (ab49881) at 0.5 µg/ml
Lane 1 : recombinant DHFR at 0.2 µg Lane 2 : recombinant DHFR at 0.1 µg Lane 3 : recombinant DHFR at 0.05 µg Lane 4 : recombinant DHFR at 0.025 µg Lane 5 : recombinant DHFR at 0.0125 µg
Developed using the ECL technique
Predicted band size : 21 kDa Observed band size : 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dihydrofolate reductase (DHFR) antibody (ab49881)This image is courtesy of an anonymous abreview.
ab49881 at a 1/30 dilution staining Dihydrofolate reductase (DHFR) in human squamous cell carcinoma tissue by Immunohistochemistry/ Formalin fixed, paraffin embedded, incubated for 12 hours at 4°C. Heat mediated antigen retrieval step performed using EDTA pH 9.0. Blocked with 1% milk for 30 minutes at 25°C. Secondary used undiluted polyclonal Goat anti-rabbit IgG conjugated to biotin.
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