Western blot - Dihydrofolate reductase (DHFR) antibody (ab49881)
All lanes : Anti-Dihydrofolate reductase (DHFR) antibody (ab49881) at 0.5 µg/ml
Lane 1 : recombinant DHFR at 0.2 µg Lane 2 : recombinant DHFR at 0.1 µg Lane 3 : recombinant DHFR at 0.05 µg Lane 4 : recombinant DHFR at 0.025 µg Lane 5 : recombinant DHFR at 0.0125 µg
developed using the ECL technique
Predicted band size : 21 kDa Observed band size : 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dihydrofolate reductase (DHFR) antibody (ab49881)This image is courtesy of an anonymous abreview.
ab49881 at a 1/30 dilution staining Dihydrofolate reductase (DHFR) in human squamous cell carcinoma tissue by Immunohistochemistry/ Formalin fixed, paraffin embedded, incubated for 12 hours at 4°C. Heat mediated antigen retrieval step performed using EDTA pH 9.0. Blocked with 1% milk for 30 minutes at 25°C. Secondary used undiluted polyclonal Goat anti-rabbit IgG conjugated to biotin.
Anderson DD et al. Serine hydroxymethyltransferase anchors de novo thymidylate synthesis pathway to nuclear lamina for DNA synthesis. J Biol Chem287:7051-62 (2012).
Read more (PubMed: 22235121) »
Martin SA et al. Methotrexate induces oxidative DNA damage and is selectively lethal to tumour cells with defects in the DNA mismatch repair gene MSH2. EMBO Mol Med1:323-37 (2009).
Read more (PubMed: 20049736) »