Anti-Digoxigenin 抗体 [HRP.21H8] (HRP) (ab6212)

製品の概要

  • 製品名Anti-Digoxigenin antibody [HRP.21H8] (HRP)
    Digoxigenin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HRP.21H8] to Digoxigenin (HRP)
  • 標識HRP
  • アプリケーション適用あり: WB, ELISA, IHC-P, IHC-Fr, ICC/IF, In situ hybridizationmore details

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.01% Thimerosal
    Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Purified anti-digoxigenin peroxidase conjugate.
  • ポリ/モノモノクローナル
  • クローン名HRP.21H8
  • ミエローマunknown
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/5000.
ELISA 1/1000 - 1/10000.
IHC-P 1/200 - 1/2000. Use in combination with tyramide signal amplification.
IHC-Fr 1/200 - 1/2000. 1/200 - 1/2000. Use in combination with tyramide signal amplification.
ICC/IF Use at an assay dependent concentration. PubMed: 22393045
In situ hybridization Use at an assay dependent dilution. PubMed: 20019669

ターゲット情報

  • 関連性Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
  • 別名
    • 1672-46-4 antibody
    • BRN 0096479 antibody
    • DIG antibody
    • EINECS 216-806-2 antibody
    • HSDB 7108 antibody
    • Lanadigenin antibody
    • Lanadigigenin antibody
    • ST056392 antibody
    see all

Anti-Digoxigenin antibody [HRP.21H8] (HRP) 画像

  • ab6212 at a dilution of 1/1000 staining Digoxigenin from human pancreatic tissue sections by immunohistochemistry (frozen). The antibody was incubated with the tissue for 2 hours.

    This image is courtesy of an Abreview submitted by an anonymous researcher on 9 December 2005.

    See Abreview

Anti-Digoxigenin antibody [HRP.21H8] (HRP) (ab6212) 使用論文

This product has been referenced in:
  • Scott H  et al. MiR-3120 is a mirror microRNA that targets heat shock cognate protein 70 and auxilin messenger RNAs and regulates clathrin vesicle uncoating. J Biol Chem 287:14726-33 (2012). ICC/IF . Read more (PubMed: 22393045) »
  • Havekes R  et al. Gravin orchestrates protein kinase A and ß2-adrenergic receptor signaling critical for synaptic plasticity and memory. J Neurosci 32:18137-49 (2012). Read more (PubMed: 23238728) »

See all 9 Publications for this product

Product Wall

Thank you for contacting us.

I have attached the CoC for ab6212 to this email.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our pr...

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Thanks so much for sending the protocol, data and schematic image. That is all very helpful to see.

From previous email, it was noted that lot GR40978-1 produced a linear curve while lots GR44124-3, GF63837-3 and GR63837-4 produced a plateau ...

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Thank you for your enquiry.

Regarding lots GR62763-3 and GR62763-4 for ab6212: These are treated as 2 different lots in our stock system. However we receive this antibody from an outside lab and, from this lab, GR62763-3 and GR62763-4 are not...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Pancreas)
Specification Pancreas
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Abcam user community

Verified customer

投稿 Jun 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Mar 31 2009

Thank you for your patience in this matter. I have received a reply from the originator of ab6212. The concentration of this product is lot dependent, usually between 0.2 - 0.5 mg/ml. The concentration of our present lot is ~0.4 mg/ml. Please d...

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Thank you for your enquiry. I have received word from the originator that they have not specifically tested this product in in situ hybridization. Please see their response below: This product was used successfully in IHC at the dilution of 1/10...

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Thank you for your patience. Regarding ab6212 (Digoxigenin antibody [HRP.21H8]), here is the requested information: antibody concentration: 0.3 mg/ml immunogen: digoxigenin coupled to KLH (details unavailable) I hope this information helps. ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (in situ hybridization in pancreas)
Specification in situ hybridization in pancreas
Fixative Paraformaldehyde
Blocking step Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1
Username

Abcam user community

Verified customer

投稿 Dec 09 2005

Thank you for your recent enquiry regarding ab6212. The concentration of the lot 104388 is 0.43 mg/ml. Please let me know if you require further information,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"