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Immunogen was a synthetic peptide, which represented a portion of human diaphanous homolog 2 encoded within exon 3 (LocusLink ID 1730).
Our Abpromise guarantee covers the use of ab12319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 132 kDa.|
Detection of Human Diaphanous 2 by Western Blot.
Samples: Whole cell lysate (50 mcg) from HEK 293T cells.
Antibody: ab12319 used at the indicated concentrations.
ab12319 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"