Anti-DIAPH2 抗体 (ab12319)


  • 製品名Anti-DIAPH2 antibody
    DIAPH2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DIAPH2
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human diaphanous homolog 2 encoded within exon 3 (LocusLink ID 1730).

  • ポジティブ・コントロール
    • HEK 293T cells.



Our Abpromise guarantee covers the use of ab12319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 132 kDa.


  • 機能Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
  • 組織特異性Expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes.
  • 関連疾患Defects in DIAPH2 are the cause of premature ovarian failure type 2A (POF2A) [MIM:300511]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • 配列類似性Belongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • 発生段階Expressed from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles.
  • ドメインDRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
  • 細胞内局在Cytoplasm > cytosol. Early endosome. Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dia 2 antibody
    • DIA antibody
    • Dia drome antibody
    • Dia2 antibody
    • Diap 2 antibody
    • Diap2 antibody
    • DIAP2_HUMAN antibody
    • DIAPH 2 antibody
    • Diaph2 antibody
    • Diaphanous 2 antibody
    • Diaphanous homolog 2 (Drosophila) antibody
    • Diaphanous homolog 2 antibody
    • Diaphanous related formin 2 antibody
    • Diaphanous-related formin-2 antibody
    • Diaphanous2 antibody
    • Diaphorase 2 antibody
    • Diaphorase2 antibody
    • DRF 2 antibody
    • DRF2 antibody
    • FLJ11167 antibody
    • OTTHUMP00000024270 antibody
    • OTTHUMP00000024271 antibody
    • OTTHUMP00000062171 antibody
    • POF 2 antibody
    • POF antibody
    • POF2 antibody
    • Protein diaphanous homolog 2 antibody
    see all

Anti-DIAPH2 antibody 画像

  • Predicted band size : 132 kDa

    Detection of Human Diaphanous 2 by Western Blot.
    Samples: Whole cell lysate (50 mcg) from HEK 293T cells.
    Antibody: ab12319 used at the indicated concentrations.
    Detection: Chemiluminescence.
  • IHC image of ab12319 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12319, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-DIAPH2 antibody (ab12319) 使用論文

ab12319 has not yet been referenced specifically in any publications.

Product Wall

We have added the blocking peptides for the two DIAPH2 antibodies you asked about to our catalogue.

DIAPH2 peptide (ab156989) for ab12319
DIAPH2 peptide (ab156990) for ab102841

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human Vein Endothelial Cells)
Loading amount 50 µg
Specification Human Vein Endothelial Cells
Gel Running Conditions Reduced Denaturing (7.5 %)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Mrs. Elena Astanina

Verified customer

投稿 Feb 23 2012

Thank you for your patience in this matter. I have received a reply from the originator of ab12319 (DIAPH2 antibody). The immunogenic peptide sequence is proprietary information. However, they indicated that this antibody recognizes an epitope tha...

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