製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DIAPH2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Human
  • 免疫原

    Synthetic peptide conjugated to KLH corresponding to C terminal amino acids of Mouse DIAPH2.

  • ポジティブ・コントロール
    • A431, HeLa, Mouse brain tissue, C2C12 cells, Rat PC12 and Rabbit spleen fibroblast lysates.
  • 特記事項
    Do not aliquot. Stable for 1 year.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 126 kDa).

ターゲット情報

  • 機能
    Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
  • 組織特異性
    Expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes.
  • 関連疾患
    Defects in DIAPH2 are the cause of premature ovarian failure type 2A (POF2A) [MIM:300511]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • 配列類似性
    Belongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • 発生段階
    Expressed from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles.
  • ドメイン
    DRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
  • 細胞内局在
    Cytoplasm > cytosol. Early endosome. Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dia 2 antibody
    • DIA antibody
    • Dia drome antibody
    • Dia2 antibody
    • Diap 2 antibody
    • Diap2 antibody
    • DIAP2_HUMAN antibody
    • DIAPH 2 antibody
    • Diaph2 antibody
    • Diaphanous 2 antibody
    • Diaphanous homolog 2 (Drosophila) antibody
    • Diaphanous homolog 2 antibody
    • Diaphanous related formin 2 antibody
    • Diaphanous-related formin-2 antibody
    • Diaphanous2 antibody
    • Diaphorase 2 antibody
    • Diaphorase2 antibody
    • DRF 2 antibody
    • DRF2 antibody
    • FLJ11167 antibody
    • OTTHUMP00000024270 antibody
    • OTTHUMP00000024271 antibody
    • OTTHUMP00000062171 antibody
    • POF 2 antibody
    • POF antibody
    • POF2 antibody
    • Protein diaphanous homolog 2 antibody
    see all

画像

  • All lanes : Anti-DIAPH2 antibody (ab119702) at 1/500 dilution

    Lane 1 : Rat PC12 lysate
    Lane 2 : Human A431 lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Rabbit spleen fibroblast lysate


    Predicted band size : 126 kDa

プロトコール

参考文献

ab119702 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Ovary)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Ovary
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: RT°C
Fixative
Paraformaldehyde
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投稿 Apr 14 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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