製品の概要

  • 製品名Anti-DIAPH2 antibody
    DIAPH2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DIAPH2
  • 特異性This antibody detects a band at 130kD in HEK293 cells representing DIA2.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide:

    VPLERSRSRHNGAISSK

    conjugated to KLH, corresponding to amino acids 1085-1101 of Human DIA.

  • ポジティブ・コントロール
    • HEK293 whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/6000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).

ターゲット情報

  • 機能Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
  • 組織特異性Expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes.
  • 関連疾患Defects in DIAPH2 are the cause of premature ovarian failure type 2A (POF2A) [MIM:300511]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • 配列類似性Belongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • 発生段階Expressed from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles.
  • ドメインDRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
  • 細胞内局在Cytoplasm > cytosol. Early endosome. Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dia 2 antibody
    • DIA antibody
    • Dia drome antibody
    • Dia2 antibody
    • Diap 2 antibody
    • Diap2 antibody
    • DIAP2_HUMAN antibody
    • DIAPH 2 antibody
    • Diaph2 antibody
    • Diaphanous 2 antibody
    • Diaphanous homolog 2 (Drosophila) antibody
    • Diaphanous homolog 2 antibody
    • Diaphanous related formin 2 antibody
    • Diaphanous-related formin-2 antibody
    • Diaphanous2 antibody
    • Diaphorase 2 antibody
    • Diaphorase2 antibody
    • DRF 2 antibody
    • DRF2 antibody
    • FLJ11167 antibody
    • OTTHUMP00000024270 antibody
    • OTTHUMP00000024271 antibody
    • OTTHUMP00000062171 antibody
    • POF 2 antibody
    • POF antibody
    • POF2 antibody
    • Protein diaphanous homolog 2 antibody
    see all

Anti-DIAPH2 antibody 画像



  • Predicted band size : 132 kDa


    Western blot using ab10562 on HEK293 whole cell lysate at 1/500. Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000) Exposure time: 1 minute Expected molecular weight: 132 kDa 20µg of cell lysate per lane

Anti-DIAPH2 antibody (ab10562) 使用論文

ab10562 has not yet been referenced specifically in any publications.

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Thank you for your recent e-mail. I have looked into your concerns very carefully and agree with you that the terminology can be very confusing. Following a BLAST search with the immunogen I can confirm the following: The immunogen sequence used t...

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