製品の概要

  • 製品名Anti-DIAPH1 antibody
    DIAPH1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DIAPH1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Recombinant fragment, corresponding to a sequence within amino acids 1-207 of Human DIAPH1

  • ポジティブ・コントロール
    • H1299 and Raji whole cell lysates. HeLa cells

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab96784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 141 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
  • 組織特異性Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea.
  • 関連疾患Defects in DIAPH1 are the cause of deafness autosomal dominant type 1 (DFNA1) [MIM:124900]. DFNA1 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • 配列類似性Belongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • ドメインDRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction (By similarity). DCAF7 binds to the FH2 (formin homology 2) domain.
  • 細胞内局在Cell membrane. Cell projection > ruffle membrane. Cytoplasm > cytoskeleton. Membrane ruffles, especially at the tip of ruffles, of motile cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DIAPH1 antibody
    • deafness, autosomal dominant 1 antibody
    • DFNA1 antibody
    • DIA1 antibody
    • DIAP1 antibody
    • DIAP1_HUMAN antibody
    • DIAPH1 antibody
    • Diaphanous homolog 1 (Drosophila) antibody
    • diaphanous homolog 1 antibody
    • Diaphanous related formin 1 antibody
    • Diaphanous-related formin-1 antibody
    • DRF1 antibody
    • FLJ25265 antibody
    • hDIA1 antibody
    • LFHL1 antibody
    • low frequency hearing loss 1 antibody
    • p140DIA antibody
    • Protein diaphanous homolog 1 antibody
    see all

Anti-DIAPH1 antibody 画像

  • All lanes : Anti-DIAPH1 antibody (ab96784) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 141 kDa
  • ab96784 at 1/200 dilution staining DIAPH1 in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is co-stained with Hoechst 33342.

Anti-DIAPH1 antibody (ab96784) 使用論文

ab96784 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Pig Cell lysate - whole cell (heart)
Gel Running Conditions Reduced Denaturing
Loading amount 5 µg
Specification heart
Blocking step Licor as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
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投稿 Nov 04 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"