製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DIAPH1
  • アプリケーション
    適用あり: ICC/IF, IHC-Fr, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, which represents a portion of the human diaphanous homolog 1 encoded within exon 27 (LocusLink ID 1729).

  • ポジティブ・コントロール
    • Tested with 293T cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antibodies were affinity purified using the peptide immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/400. PubMed: 24302570
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000 - 1/15000. Detects a band of approximately 200 kDa.

ターゲット情報

  • 機能
    Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
  • 組織特異性
    Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea.
  • 関連疾患
    Defects in DIAPH1 are the cause of deafness autosomal dominant type 1 (DFNA1) [MIM:124900]. DFNA1 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • 配列類似性
    Belongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • ドメイン
    DRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction (By similarity). DCAF7 binds to the FH2 (formin homology 2) domain.
  • 細胞内局在
    Cell membrane. Cell projection > ruffle membrane. Cytoplasm > cytoskeleton. Membrane ruffles, especially at the tip of ruffles, of motile cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DIAPH1 antibody
    • deafness, autosomal dominant 1 antibody
    • DFNA1 antibody
    • DIA1 antibody
    • DIAP1 antibody
    • DIAP1_HUMAN antibody
    • DIAPH1 antibody
    • Diaphanous homolog 1 (Drosophila) antibody
    • diaphanous homolog 1 antibody
    • Diaphanous related formin 1 antibody
    • Diaphanous-related formin-1 antibody
    • DRF1 antibody
    • FLJ25265 antibody
    • hDIA1 antibody
    • LFHL1 antibody
    • low frequency hearing loss 1 antibody
    • p140DIA antibody
    • Protein diaphanous homolog 1 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DIAPH1 with ab11173 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : ab11173 diluted 1/5000

    Lane 1 : African Green Monkey COS-7 whole cell lysate with 5% Milk
    Lane 2 : African Green Monkey COS-7 whole cell lysate with 5% Milk

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated Goat anti-rabbit polyclonal diluted 1/4000
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 200 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 seconds

    This image is courtesy of an anonymous Abreview

    The gel running conditions were reducing.  The blocking time was 2 hours at 25°C.

    The primary antibody was incubated for 16 hours at 4°C.

    See Abreview

  • IHC image of ab11173 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11173, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献

This product has been referenced in:
  • Lin YN  et al. Drosophila homologue of Diaphanous 1 (DIAPH1) controls the metastatic potential of colon cancer cells by regulating microtubule-dependent adhesion. Oncotarget 6:18577-89 (2015). Read more (PubMed: 26124177) »
  • Araki K  et al. Cytoplasmic translocation of the retinoblastoma protein disrupts sarcomeric organization. Elife 2:e01228 (2013). IHC-Fr ; Human . Read more (PubMed: 24302570) »

See all 3 Publications for this product

レビューと Q&A

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Vein Endothelial Cells)
Loading amount
50 µg
Specification
Human Vein Endothelial Cells
Gel Running Conditions
Reduced Denaturing (7.5 %)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Mrs. Elena Astanina

Verified customer

投稿 Feb 23 2012

Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (COS-7)
Loading amount
20 µg
Specification
COS-7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 23 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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