製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DHX8
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Tilapia, Chinese hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 325 and 375 of Human DHX8 (NP_004932.1)

  • ポジティブ・コントロール
    • Human gastric tumor, Mouse squamous cell carcinoma

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報
    IHC-P: 1/100 - 1/500.
    Epitope exposure is recommended.
    Epitope exposure with citrate buffer will enhance staining.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.
    • 配列類似性
      Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 S1 motif domain.
    • ドメイン
      The RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATP dependent RNA helicase DHX8 antibody
      • ATP-dependent RNA helicase dhx8 antibody
      • DDX 8 antibody
      • DDX8 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 8 (RNA helicase) antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 8 antibody
      • DEAD/H box 8 antibody
      • DEAD/H box polypeptide 8 antibody
      • DEAH (Asp Glu Ala His) box polypeptide 8 antibody
      • DEAH box polypeptide 8 antibody
      • DEAH box protein 8 antibody
      • DHX 8 antibody
      • dhx8 antibody
      • DHX8_HUMAN antibody
      • HRH 1 antibody
      • HRH1 antibody
      • PRP 22 antibody
      • PRP22 antibody
      • PRP22, S. cerevisiae, homolog of antibody
      • PRPF 22 antibody
      • PRPF22 antibody
      • RNA helicase HRH 1 antibody
      • RNA helicase HRH1 antibody
      see all

    画像

    • Detection of Human DHX8 by Immunohistochemistry on paraffin embedded formalin fixed section of Human gastric tumor using 1/500 ab84505. Detection: DAB staining
    • Detection of Mouse DHX8 by Immunohistochemistry on paraffin embedded formalin fixed section of Mouse squamous cell carcinoma using 1/100 ab84505. Detection: DAB staining

    プロトコール

    参考文献

    ab84505 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Fruit fly (Drosophila melanogaster) Cell (Egg chamber)
    Specification
    Egg chamber
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    BSA + goat serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Username

    Mr. Stephen Klusza

    Verified customer

    投稿 Jul 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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