Rabbit polyclonal to DHX8
Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Tilapia, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to a region between residues 325 and 375 of human DHX9. (NP_004932.1)
Whole cell lysate from HeLa and 293T cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
Use at 1-4 µg/mg of lysate.
Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.
Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.
Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 S1 motif domain.
The RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome.
Information by UniProt
ATP dependent RNA helicase DHX8 antibody
ATP-dependent RNA helicase dhx8 antibody
DDX 8 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX8 antibody (ab70588)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma (right) tissues labelling DHX8 with ab70588 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Anti-DHX8 antibody (ab70588)
All lanes :
Anti-DHX8 antibody (ab70588) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Predicted band size:
Observed band size:
150 kDa (
why is the actual band size different from the predicted?
Additional bands at:
<30 kDa, 117 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - DHX8 antibody (ab70588)
Immunoprecipitation of DHX8 from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab70588 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent WB detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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