製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to DHX58
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    DFLQHCAENLSD

    , corresponding to C terminal amino acids 663-674 of Human DHX58 (NP_077024.2)

  • ポジティブ・コントロール
    • Human liver lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/8000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 77 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Participates in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade which results in the induction and expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). The RNA helicase domain may recognize and structurally modify viral RNA to facilitate detection by DDX58/RIG-I or by IFIH1/MDA5 whose affinity for dsRNA is lower.
    • 配列類似性
      Belongs to the helicase family.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • D11LGP2 antibody
      • D11lgp2e antibody
      • DEXH (Asp Glu X His) box polypeptide 58 antibody
      • DEXH box polypeptide 58 antibody
      • DHX 58 antibody
      • DHX58 antibody
      • DHX58_HUMAN antibody
      • LGP 2 antibody
      • LGP2 antibody
      • Ortholog of mouse D11lgp2 antibody
      • Probable ATP dependent helicase LGP2 antibody
      • Probable ATP dependent RNA helicase DHX58 antibody
      • Probable ATP-dependent helicase LGP2 antibody
      • Probable ATP-dependent RNA helicase DHX58 antibody
      • Protein D11Lgp2 homolog antibody
      • RIG-I-like receptor LGP2 antibody
      • RLR antibody
      • RNA helicase LGP2 antibody
      see all

    画像

    • Anti-DHX58 antibody (ab82151) at 0.3 µg/ml + Human liver lysate in RIPA buffer at 35 µg

      Predicted band size : 77 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.

    プロトコール

    参考文献

    ab82151 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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