The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
Use a concentration of 10 µg/ml.
Participates in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade which results in the induction and expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). The RNA helicase domain may recognize and structurally modify viral RNA to facilitate detection by DDX58/RIG-I or by IFIH1/MDA5 whose affinity for dsRNA is lower.
Belongs to the helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
ICC/IF image of ab67270 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67270, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-DHX58 antibody (ab67270)
Lane 1 : Anti-DHX58 antibody (ab67270) at 1 µg/ml Lane 2 : Anti-DHX58 antibody (ab67270) at 2 µg/ml
All lanes : Rat kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary All lanes : Anti rabbit secondary antibody
Predicted band size: 77 kDa Observed band size: 77 kDa Additional bands at: 80 kDa, 90 kDa. We are unsure as to the identity of these extra bands.