The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
DHX37 is a member of the family of the DEAD box proteins, a group of proteins that present the conserved motif Asp-Glu-Ala-Asp (DEAD). They have a role in RNA translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.
Probable ATP dependent RNA helicase DHX37 antibody
Western blot - DHX37 antibody (ab70778)
All lanes : Anti-DHX37 antibody (ab70778) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Detection of DHX37 by Western Blot of Immunprecipitate.
ab70778 at 1µg/ml staining DHX37 in HeLa whole cell lysate immunoprecipitated using ab70778 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.