Anti-DHX36 抗体 (ab70269)
Key features and details
- Rabbit polyclonal to DHX36
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DHX36 antibody
DHX36 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DHX36 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rhesus monkey, Gorilla, Orangutan -
免疫原
A region corresponding to C terminal amino acids 950-1008 of Human DHX36 (NP_065916.1)
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ポジティブ・コントロール
- WB: HeLa, HEK293T, Jurkat, mouse NIH3T3, TCKM, Renca whole cell lysates; IHC: human testicular seminoma, human testis;
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70269の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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WB | (1) |
1/5000 - 1/15000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/5000 - 1/15000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. May function in sex development and spermatogenesis. -
組織特異性
Highly expressed in testis. -
配列類似性
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
細胞内局在
Nucleus. Cytoplasm. Isoform 1 preferentially localized to the nucleus and isoform 2 localized to the cytoplasm. However, partitioning of cellular localization between the nucleus and cytoplasm is not exclusive, as isoform 1 was also detected in the cytoplasm. Both isoforms were excluded from nucleoli. - Information by UniProt
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参照データベース
- Entrez Gene: 170506 Human
- Entrez Gene: 72162 Mouse
- Omim: 612767 Human
- SwissProt: Q9H2U1 Human
- SwissProt: Q8VHK9 Mouse
- Unigene: 446270 Human
- Unigene: 224233 Mouse
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別名
- DDX36 antibody
- DEAH box protein 36 antibody
- Dhx36 antibody
see all
画像
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All lanes : Anti-DHX36 antibody (ab70269) at 1 µg/ml
Lane 1 : NIH/3T3 whole cell lysate
Lane 2 : TCKM whole cell lysate
Lane 3 : Renca whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 115 kDa -
All lanes : Anti-DHX36 antibody (ab70269) at 0.4 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HEK293T cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Predicted band size: 115 kDa -
Immunoprecipitation of HeLa whole cell lysate using ab70269
Lane 1: current lot ab70269
Lane 2: previous lot ab70269
Lane 3: Control IgG
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling DHX36 with ab70269 at 1/1000 (1µg/ml). Detection: DAB.
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IHC image of ab70269 staining in Human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70269, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab70269 は 12 報の論文で使用されています。
- Liu H et al. A Helicase Unwinds Hexanucleotide Repeat RNA G-Quadruplexes and Facilitates Repeat-Associated Non-AUG Translation. J Am Chem Soc 143:7368-7379 (2021). PubMed: 33855846
- He L et al. DNA sensors, crucial receptors to resist pathogens, are deregulated in colorectal cancer and associated with initiation and progression of the disease. J Cancer 11:893-905 (2020). PubMed: 31949493
- Herviou P et al. hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma. Nat Commun 11:2661 (2020). PubMed: 32461552
- Murat P et al. RNA G-quadruplexes at upstream open reading frames cause DHX36- and DHX9-dependent translation of human mRNAs. Genome Biol 19:229 (2018). PubMed: 30591072
- Remenyi J et al. The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132. Sci Rep 6:22848 (2016). PubMed: 26947125