All lanes : Anti-DHX30 antibody (ab85687) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg
Predicted band size: 134 kDa Observed band size: 134 kDa Additional bands at: 110 kDa, 238 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - DHX30 antibody (ab85687)
Detection of Human DHX30 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85687 at 10 ug/mg lysate. Subsequent Western blot analysis was perfomed using 0.4 ug/ml ab85687.