製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DHX15
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • 免疫原

    Synthetic peptide corresponding to a region between C terminal residues 750-795 of human DHX15 (Swiss-Prot O43143).

  • ポジティブ・コントロール
    • 293T whole cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab70454 was affinity purified using an epitope specific to DHX15 immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab70454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/15000. Detects a band of approximately 97 kDa (predicted molecular weight: 91 kDa).
IP Use at 1-5 µg/mg of lysate.

ターゲット情報

画像

  • All lanes : Anti-DHX15 antibody (ab70454) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from 293T cells.
    Lane 2 : Whole cell lysate from 293T cells.

    Lysates/proteins at 60 µg per lane.


    Predicted band size : 91 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
  • 1 mg 293T whole cell lysate. ab70454 used at 0.1 µg/ml for the Western blot and at 1 µg/mg lysate for Immunoprecipitation.

参考文献

This product has been referenced in:
  • Chen YL  et al. The telomerase inhibitor Gno1p/PINX1 activates the helicase Prp43p during ribosome biogenesis. Nucleic Acids Res 42:7330-45 (2014). WB ; Human . Read more (PubMed: 24823796) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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