Detection of Human DHX 15 by Western Blot. Samples: Whole cell lysate (55 mcg/lane) from human lymphoblasts. Antibody: Affinity purified rabbit anti-DHX15 antibody 13460 used at 0.04 mcg/ml. Detection: Chemiluminescence with exposure times of 1 and 5 minutes.
Anti-STAT1 antibody (ab26859) at 1/2000 dilution + STAT1 Ecoli derived fusion protein