製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DHODH
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide, corresponding to a region within internal sequence amino acids 179-228 (NLGKNKTSVD AAEDYAEGVR VLGPLADYLV VNVSSPNTAG LRSLQGKAEL) of Human DHODH (NP_001352)

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

ターゲット情報

  • 機能
    Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
  • パスウェイ
    Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
  • 関連疾患
    Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases.
  • 配列類似性
    Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
  • 翻訳後修飾
    The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.
  • 細胞内局在
    Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DHOdehase antibody
    • Dhodh antibody
    • Dihydroorotate dehydrogenase (quinone) antibody
    • Dihydroorotate dehydrogenase antibody
    • Dihydroorotate dehydrogenase mitochondrial antibody
    • Dihydroorotate oxidase antibody
    • Human complement of yeast URA1 antibody
    • mitochondrial antibody
    • POADS antibody
    • PYRD_HUMAN antibody
    • URA1 antibody
    see all

画像

  • Anti-DHODH antibody (ab83457) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab83457 has not yet been referenced specifically in any publications.

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