製品の概要

  • 製品名
    Anti-Dhh antibody [RM0086-6A12]
    Dhh 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [RM0086-6A12] to Dhh
  • 特異性
    ab86061 detects Mouse Dhh N terminal polypeptide, but not C terminal polypeptide.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Mouse recombinant Dhh N-terminal fragment

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and filter sterilized through a 0.2 µm filter.
  • ポリ/モノ
    モノクローナル
  • クローン名
    RM0086-6A12
  • アイソタイプ
    IgG2
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab86061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: 1/500 - 1/1000. Predicted molecular weight: Unprocessed precursor, 44 kDa; Dhh protein N-product, 20 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.
    • 関連疾患
      Defects in DHH may be the cause of partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD) [MIM:607080]. PGD is characterized by the presence of a testis on one side and a streak or an absent gonad at the other, persistence of Muellerian duct structures, and a variable degree of genital ambiguity.
      Defects in DHH may be the cause of complete pure gonadal dysgenesis 46,XY type (GDXYM) [MIM:233420]; also known as male-limited gonadal dysgenesis 46,XY. GDXYM is a type of hypogonadism in which no functional gonads are present to induce puberty in an externally female person whose karyotype is then found to be XY. The gonads are found to be non-functional streaks.
    • 配列類似性
      Belongs to the hedgehog family.
    • 翻訳後修飾
      The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). This covalent modification appears to play an essential role in restricting the spatial distribution of the protein activity to the cell surface. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
    • 細胞内局在
      Secreted > extracellular space. The C-terminal peptide diffuses from the cell and Cell membrane. The N-terminal peptide remains associated with the cell surface.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C78960 antibody
      • Desert hedgehog (Drosophila) homolog antibody
      • Desert hedgehog antibody
      • Desert hedgehog homolog (Drosophila) antibody
      • Desert hedgehog protein C-product antibody
      • Desert hedgehog protein precursor antibody
      • DHH antibody
      • DHH_HUMAN antibody
      • GDXYM antibody
      • HHG-3 antibody
      • Hira antibody
      • SRXY7 antibody
      see all

    プロトコール

    参考文献

    This product has been referenced in:
    • Daane JM & Downs KM Hedgehog signaling in the posterior region of the mouse gastrula suggests manifold roles in the fetal-umbilical connection and posterior morphogenesis. Dev Dyn 240:2175-93 (2011). Read more (PubMed: 22016185) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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