製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Dhh
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 135-388 of Human Dhh (NP_066382)

  • ポジティブ・コントロール
    • 293T; Jurkat or Raji lysates and A431 whole cell lysate.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.

ターゲット情報

  • 機能
    Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.
  • 関連疾患
    Defects in DHH may be the cause of partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD) [MIM:607080]. PGD is characterized by the presence of a testis on one side and a streak or an absent gonad at the other, persistence of Muellerian duct structures, and a variable degree of genital ambiguity.
    Defects in DHH may be the cause of complete pure gonadal dysgenesis 46,XY type (GDXYM) [MIM:233420]; also known as male-limited gonadal dysgenesis 46,XY. GDXYM is a type of hypogonadism in which no functional gonads are present to induce puberty in an externally female person whose karyotype is then found to be XY. The gonads are found to be non-functional streaks.
  • 配列類似性
    Belongs to the hedgehog family.
  • 翻訳後修飾
    The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). This covalent modification appears to play an essential role in restricting the spatial distribution of the protein activity to the cell surface. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
  • 細胞内局在
    Secreted > extracellular space. The C-terminal peptide diffuses from the cell and Cell membrane. The N-terminal peptide remains associated with the cell surface.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C78960 antibody
    • Desert hedgehog (Drosophila) homolog antibody
    • Desert hedgehog antibody
    • Desert hedgehog homolog (Drosophila) antibody
    • Desert hedgehog protein C-product antibody
    • Desert hedgehog protein precursor antibody
    • DHH antibody
    • DHH_HUMAN antibody
    • GDXYM antibody
    • HHG-3 antibody
    • Hira antibody
    • SRXY7 antibody
    see all

画像

  • Anti-Dhh antibody (ab97287) at 1/10000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 44 kDa

プロトコール

参考文献

ab97287 has not yet been referenced specifically in any publications.

レビューと Q&A

Vielen Dank für Ihre Anfrage. In der Tat konnte ich in unserer Datenbank nichts darüber finden, dass die genannten Antikörper nicht auch für die Durchflußzytometrie geeignet sein könnten. Ich habe Ihnen daher die entsprechenden Gutscheincodes ausgestel...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録